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KMID : 0380319690020000063
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Journal of Korean Research Institute for Better Living
1969 Volume.2 No. 0 p.63 ~ p.68
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Serumless Medium for Cultivation of Chick Embryo Cells
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Abstract
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Animal serum has been a necessary constituent of most media for continous cell cultures. However, many sera contain serum antibodies and other serum inhibitors of microorganisms. It would be desirable to replace serum with another substance which does not contain such inhibitors and which would sustain cells in a condition sensitive to viral effects during the necessary observation periods. Such cells might also be useful in the sutdies of nutrutional factors in tissue culture and in evaluating the effects of of heterologous sera on cells in culture. The present report concerns the growth of chick embryo cells in tissue culture media devoid of serum, which media include casamino acid, Tween 80, pvp, gelatin, vitamin B_12 and tryptose as supplements in Hanks balnced solution plus 0.5% lactalbumin hydrolysate. Each tube was inoculated with 4.3X10^6 clels per 1.0ml of the media. Growth was measured by the method of protein estimation with the Folin phenol reagent. The results of these experiments are as following.
(1) (0.1%) Gelatin stimulated the growth rate of chick embryo cells considerably in a basal medium which consisted of Hanks BSS and 0.5% lactalbumin hydrolysate
(2) In a medium in whith 0.1% gelatin and 0.05% yeast extract, or 0.1% lactalbumin hydrolysate in Hanks BSS chick embryo cells grew more rapidly than in the medium added with only 0.1% gelatin. In these media cells increased approximately 1.3 times after 3 days and 4 days of cultivation. A monolayer sheet of epithelium like cells was formed after 5 days of cultivation.
(3) The gelatin medium added with tryptose, casamino acid. Tween 80, and pvp respectively did not gain any advantage over the original gelatin medium.
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